Journal: PloS one

Article Title: Association of interleukin-6 signalling with the muscle stem cell response following muscle-lengthening contractions in humans.

doi: 10.1371/journal.pone.0006027

Figure Lengend Snippet: Figure 1. Circulating IL-6 and muscle IL-6 mRNA and IL-6 receptor response muscle lengthening contractions (MLC). (1a) Average serum interleukin-6 (IL-6) concentration. Time 0 hr corresponds to pre-intervention values; all other time-points correspond to post-intervention time (hr). (1b) Relative IL-6 mRNA expression, expressed as fold-change from 0 hr. (1c) Pearson correlation of serum IL-6 concentration versus muscle IL-6 mRNA (fold-change), correlation is representative of the individual data points and presented as mean values ($)6SD (error bars). (1d) Relative IL-6 receptor (IL-6Ra) mRNA expression, expressed as fold-change from 0 hr. (1e) Immunofluorescent image (406) of a muscle cross-section triple-stained for Pax7 (red), IL-6Ra (green) and nuclei (DAPI = blue); IL-6Ra staining is apparent on the sarcolemma and satellite cell membrane. White arrow denotes one of the Pax7+ nuclei which co-localized with IL-6Ra (scale bar = 100 mm). Values are reported as mean6S.E.M. Mean values represent the mean for all 8 subjects per time-point (8 samples per time-point, 40 samples total). *p,0.05 vs. 0 hr. doi:10.1371/journal.pone.0006027.g001

Article Snippet: Immunofluorescence 7 mm sections were cryosectioned and stained with antibodies against Pax7 (neat; DSHB, USA); IL-6 (500 ng/mL, MAB 2061, R&D Systems, USA); p-STAT3 (p-STAT3 Y705 1:100, Cell Signaling Technologies Inc., USA); IL-6Ra (1:50, MCA822, Serotec, UK); PCNA (1:200, ab15497, Abcam Inc., USA); and Laminin (1:1000, L8271, Sigma-Aldrich, Canada).

Techniques: Concentration Assay, Expressing, Staining, Membrane

Journal: mAbs

Article Title: Discovery and characterization of a monoclonal antibody targeting a conformational epitope of IL-6/IL-6Rα to inhibit IL-6/ IL-6Rα/gp130 hexameric signaling complex formation

doi: 10.1080/19420862.2022.2029675

Figure Lengend Snippet:

Article Snippet: In addition, IL-6 (R&D systems, #7270-IL/CF), IL-6Rα (R&D systems, #227-SR/CF), human IL-6Rα/IL-6 chimera (R&D systems, #8954-SR/CF), and mouse IL-6/IL-6Rα complex (R&D systems, #9038-SR/CF) were used for ELISA, cell function, in vivo model, and kinetics assays.

Techniques: Modification, Saline, Enzyme-linked Immunosorbent Assay, Mass Spectrometry, Liquid Chromatography, Phospho-proteomics, Nucleic Acid Electrophoresis

Journal: Cell Reports Medicine

Article Title: Integrated immune dynamics define correlates of COVID-19 severity and antibody responses

doi: 10.1016/j.xcrm.2021.100208

Figure Lengend Snippet:

Article Snippet: Patient’s plasma was diluted 1:2 for measuring IL-1β, IFN-α2, IFNγ, TNFα, MCP-1 (CCL2), IL-6, IL-8 (CXCL8), IL-10, IL-12p70, IL-17A, IL-18, IL-23 and IL-33 using the LEGENDplex Human Inflammation Panel 1 kit, according to manufacturer’s instructions (BioLegend, San Diego, CA, USA). sIL-6R levels were measured in plasma (diluted 1:300) using the Human IL-6R alpha DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions.

Techniques: Virus, Clinical Proteomics, Control, Recombinant, Enzyme-linked Immunosorbent Assay, Staining, Software